P. Khandelwal et al., RESOLUTION IMPROVEMENT IN NMR-SPECTRA - APPLICATION AND SIGNIFICANCE OF CHEMICAL-SHIFT UPSCALING IN MULTIDIMENSIONAL NMR EXPERIMENTS, Concepts in magnetic resonance, 10(6), 1998, pp. 355-368
An analysis of resolution in nuclear magnetic resonance (NMR) spectra
in terms of separation, linewidth, and limited digitization capability
is presented. The effect of scaling up of frequencies on resolution i
s considered, and criteria useful for determining whether two peaks ar
e resolvable in continuous-wave and digitized spectra are derived. Fin
ally, a new pulse sequence based on upscaling of chemical shifts is de
scribed for resolution improvement along the F-1 dimension in heteronu
clear correlation NMR spectra. The performance of the sequence is demo
nstrated by experimental data on large proteins, and simulations are p
erformed for its optimization. (C) 1998 John Wiley & Sons. Inc.