CAPILLARY CHROMATOGRAPHY MICROELECTROSPRAY MASS-SPECTROMETRY USED FORTHE IDENTIFICATION OF PUTATIVE CYCLIN-DEPENDENT KINASE INHIBITORY PROTEIN IN MEDICAGO

Authors
KELE Z JANAKY T MESZAROS T FEHER A DUDITS D SZABO PT
Citation
Z. Kele et al., CAPILLARY CHROMATOGRAPHY MICROELECTROSPRAY MASS-SPECTROMETRY USED FORTHE IDENTIFICATION OF PUTATIVE CYCLIN-DEPENDENT KINASE INHIBITORY PROTEIN IN MEDICAGO, Rapid communications in mass spectrometry, 12(20), 1998, pp. 1564-1568
Citations number
21
Categorie Soggetti
Spectroscopy,"Chemistry Analytical
Journal title
Rapid communications in mass spectrometry → ACNP
ISSN journal
09514198
Volume
12
Issue
20
Year of publication
1998
Pages
1564 - 1568
Database
ISI
SICI code
0951-4198(1998)12:20<1564:CCMMUF>2.0.ZU;2-4
Abstract
A home-built capillary chromatography/microelectrospray system was use d for peptide mass mapping of a putative cyclin-dependent kinase inhib itor protein with molecular mass of 8.5 kDa. The masses of identified tryptic fragments were then input to a protein database search routine . Daughter ion scans were done only on the most abundant tryptic fragm ents. On the basis of protein database search results and tandem mass spectrometric measurements the bioactive protein was identified as ubi quitin. (C) 1998 John Wiley & Sons, Ltd.