NEUROPEPTIDE-Y RECEPTORS INVOLVED IN CALCIUM-CHANNEL REGULATION IN PC12 CELLS

Our laboratory has previously used NGF-differentiated PC12 cells as a sympathetic neuronal model to investigate the effects of NPY on catech olamine synthesis and release. We have additionally used these cells t o demonstrate the NPY-induced inhibition of Ca2+ channels which was su ggested by those studies. In the present work, multiple NPY, PYY, and PP analogs are utilized to further define the receptor subtypes involv ed in this Ca2+ channel modulation. We find that in PC12 cells NPY and PP modulate Ca channels through Y1, Y2, Y3, and Y4 receptors. In addi tion, we show that these receptors are differentially coupled to N, L, and non-N, non-L Ca2+ channel subtypes. The results of the present st udy in combination with our previous investigations demonstrate an int riguing and complex role for NPY and PP in the modulation of sympathet ic neurotransmission. (C) 1998 Elsevier Science B.V. All rights reserv ed.