THE RENIN-ANGIOTENSIN SYSTEM IN HYBRID NG108-15 CELLS - RENIN GENE ISFROM MOUSE NEUROBLASTOMA, ANGIOTENSINOGEN AND ANGIOTENSIN-CONVERTING ENZYME GENES ARE OF RAT GLIOMA ORIGIN

Angiotensin II (Ang II) increases the level of tyrosine phosphorylatio n of several proteins in nondifferentiated NG108-15 cells, a hybrid de rived from the fusion of mouse neuroblastoma and rat glioma cells. Con versely, incubation of NG108-15 cells with an angiotensin-converting e nzyme (ACE) inhibitor decreased the basal level of tyrosine phosphoryl ation of proteins, suggesting that locally secreted Ang II may act as an autocrine regulator. By RT-PCR, we found that nondifferentiated NG1 08-15 cells contained the mRNA transcript of the rat angiotensinogen, mouse renin and rat ACE genes, thus confirming that NG108-15 cells con tain all the elements of a local renin-angiotensin system. (C) 1998 El sevier Science B.V. All rights reserved.