SELECTIVE EXPRESSION OF VASOACTIVE-INTESTINAL-PEPTIDE (VIP)(2) PITUITARY ADENYLATE CYCLASE-ACTIVATING POLYPEPTIDE (PACAP)(3) RECEPTORS IN RABBIT AND GUINEA-PIG GASTRIC AND TENIA COLI SMOOTH-MUSCLE CELLS/

Authors
TENG BQ MURTHY KS KUEMMERLE JF GRIDER JR MAKHLOUF GM
Citation
Bq. Teng et al., SELECTIVE EXPRESSION OF VASOACTIVE-INTESTINAL-PEPTIDE (VIP)(2) PITUITARY ADENYLATE CYCLASE-ACTIVATING POLYPEPTIDE (PACAP)(3) RECEPTORS IN RABBIT AND GUINEA-PIG GASTRIC AND TENIA COLI SMOOTH-MUSCLE CELLS/, Regulatory peptides, 77(1-3), 1998, pp. 127-134
Citations number
31
Categorie Soggetti
Endocrynology & Metabolism",Physiology
Journal title
Regulatory peptides → ACNP
ISSN journal
01670115
Volume
77
Issue
1-3
Year of publication
1998
Pages
127 - 134
Database
ISI
SICI code
0167-0115(1998)77:1-3<127:SEOV(P>2.0.ZU;2-V
Abstract
In both functional and radioligand binding studies of gastric smooth m uscle from rabbit and guinea pig, vasoactive intestinal peptide (VIP) and pituitary adenylate cyclase-activating polypeptide (PACAP) show eq ual potency indicating that the receptor type is either a VIP1 /PACAP( 2) or a VIP2/PACAP(3) receptor. We have characterized the VIP/PACAP re ceptor expressed in freshly dispersed and cultured gastric and tenia c oli smooth muscle cells of rabbit and guinea pig by reverse transcript ase-polymerase chain reaction (RT-PCR), Northern analysis, and cloning of the first extracellular domain. Specific primers based on cDNA seq uences for rat VIP1/PACAP(2), VIP2/PACAP(3) and PACAP(1) receptors wer e designed spanning the first extracellular domain. A 275 base pair pr oduct corresponding to VIP2/PACAP(3) receptor was amplified by RT-PCR in muscle cells from both species. No RT-PCR product was obtained with primers for VIP2/PACAP(3) and PACAP(1) receptors. The deduced amino a cid sequences showed 90% similarity in rabbit and 77% in guinea pig to the sequence in rat. The location of the aspartate, tryptophan and gl ycine residues and all six N-terminal cysteines required for VIP bindi ng were conserved. The sequence in guinea pig tenia coli differed from that in guinea pig stomach by two amino acid residues, Phe(40) and Ph e(41). Northern analysis revealed a single 3.9 kilobase (kb) mRNA corr esponding to VIP2/PACAP(3) receptors in rabbit and a 2.1 kb mRNA in gu ineapig gastric and tenia coli muscle cells. We conclude that only VIP 2/PACAP(3) receptors are expressed in smooth muscle cells of rabbit an d guinea pig. The two amino acid difference in the sequence obtained f rom guinea pig tenia coli may reflect the distinct binding and functio nal properties of this tissue. (C) 1998 Elsevier Science B.V All right s reserved.