Imported seeds of Perilla frutescens L. (cv. Aka-Shiso and Ao-Shiso) w
ere propagated and cultivated in Egypt. The growth, herbal yield and v
olatile oil yield were recorded. The yield of volatile oil, extracted
by hydrodistillation, reached 0.17%, 0.01% and 0.16% of fresh weight o
f leaves, stem and flowers, respectively. The volatile oil of the aeri
al parts of each variety was analysed by capillary GC-MS. The hydrodis
tillates of both varieties mainly consist of perillaldehyde, caryophyl
lene oxide, limonene and caryophyllene, with perillaldehyde as the mai
n constituent (32.9% and 34.2% for Aka-Shiso and Ao-Shiso, respectivel
y). Some significant differences in the oil constituents were observed
, where caryophyllene oxide reached 23.3% in Ao-Shiso but accounted fo
r only 6.0% in Aka-Shiso. The antimicrobial activity for both oils was
studied. (C) 1998 John Wiley & Sons, Ltd.