Hp. Khng et al., THE SYNTHESIS OF SUBMICRON MAGNETIC PARTICLES AND THEIR USE FOR PREPARATIVE PURIFICATION OF PROTEINS, Biotechnology and bioengineering, 60(4), 1998, pp. 419-424
A novel one-step protocol for the preparation of sub-micron magnetic p
articles as small as 30 nm in diameter has been developed. The surface
of the par tides was functionalized with carboxyl groups (similar to
1 COOH per 15 Angstrom(2)) to facilitate the attachment of affinity li
gands. The high surface area of the resulting beads, combined with the
high density of functional groups on the surface, makes them ideally
suited for the preparative purification of proteins as was demonstrate
d by the efficient isolation of trypsin (36 mg per gram of particles)
from pancreatic extract. (C) 1998 John Wiley & Sons, Inc.