MOLECULAR ANALYSIS OF CARBON ION-INDUCED MUTATIONS IN ARABIDOPSIS-THALIANA

In order to elucidate the characteristics of the mutations induced by ion particles at the molecular level in plants, mutated loci in carbon ion-induced mutants of Arabidopsis were investigated by PCR and South ern blot analyses. In the present study, two lines of gl1 mutant and t wo lines of tt4 mutant were isolated after carbon ion-irradiation. Out of four mutants, one had a deletion, other two contained rearrangemen ts, and one had a point-like mutation. From the present result, it was suggested that ion particles induced different kinds of alterations o f the DNA and therefore they could produce various types of mutant all eles in plants.