This study describes the psychological aspects and the experience of p
ain in 2 groups of women undergoing amniocentesis in relation to the d
ifferent indication for the procedure and duration of gestation in the
respective groups. Methods and subjects: Data were collected after am
niocentesis from 100 pregnant women, 50 of whom underwent age-related
amniocentesis (14 weeks of gestation) and 50 for measurement of amniot
ic fluid insulin levels (30 weeks of gestation). A semi-structured psy
chological interview was used to collect information concerning experi
ence of pain, cognition before amniocentesis, knowledge about the proc
edure and the most distressing aspects of amniocentesis. Visual analog
scales (VAS, 0 to 10) were used to assess the degree of pain and emot
ional mood state. Results: The results showed that emotional mood stat
e before amniocentesis was fair (mean = 4.6) and increased after amnio
centesis (mean = 8.1) The degree of pain during amniocentesis was asse
ssed at a mean value of 2.9 measured on the 10-point visual analog sca
le. Fifty per cent of the sample considered the physical, the other 50
% the psychological (anxiety and uncertainty) aspects as the most dist
ressing factor of amniocentesis. Although women were given prior detai
led information about amniocentesis they overestimated the degree of p
ain, related risk factors, and duration of the medical procedure. Ther
e were no differences in the investigated variables between the two di
fferent indications for amniocentesis or the weeks of gestation. Concl
usion: The findings of the study indicate the psychological distress a
ssociated with amniocentesis, which is limited to a short period of ti
me, and is independent of the indication for amniocentesis.