ANION-EXCHANGE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ASSAY OF PLASMA-LIPOPROTEINS OF RABBITS, RATS AND MICE

A fast, accurate and precise high-performance liquid chromatographic a ssay method has been developed for plasma lipoproteins of experimental animals, rabbits, rats and mice. The method includes complete separat ion of high, low and very low density lipoproteins from one another wi thin 20 min by a DEAE-glucomannan gel using stepwise elution, and dete rmination by postcolumn reaction with an enzymatic cholesterol reagent as the total cholesterol level. The relative standard deviation of ea ch Lipoprotein assay was highly reproducible, being less than 2.0 and 2.4% for repeatability and intermediate precision, respectively. The m ethod was successfully applied to the assays of plasma lipoproteins in three species of normolipidemic and hyperlipidemic animals. (C) 1998 Elsevier Science BN. All rights reserved.