BETA(2)-ADRENERGIC AGONIST RESIDUES - SIMULTANEOUS METHYL-BORONIC ANDBUTYLBORONIC DERIVATIZATION FOR CONFIRMATORY ANALYSIS BY GAS-CHROMATOGRAPHY MASS-SPECTROMETRY

A derivatization procedure for confirmatory residue analysis of beta(2 )-agonists is described. Methyl (MBA) and butyl (BBA) boronic acids ar e simultaneously used for the derivatization of tulobuterol, mabuterol , mapenterol, salbutamol, clenproperol, clenbuterol, clenpenterol and bromobuterol by GC-MS determination. A temperature of 55 degrees C dur ing 60 min was selected as optimal temperature-time condition for simu ltaneous MBA and BBA beta(2)-agonists derivatization. It was also obse rved that stability of boronic derivatives was maintained at -20 degre es C over a period of four days. The proposed methodology was tested i n urine and it could be applied for confirmatory residue analysis of c lenbuterol-like compounds. (C) 1998 Elsevier Science B.V. All rights r eserved.