SUPPRESSION OF DNA-SYNTHESIS OF CULTURED MESOTHELIAL CELLS BY SUPPLEMENTED ALBUMIN

Mesothelial cells, harvested from rat omentum, were cultured with 10% FCS/MEM fill the third passage, and then seeded at a density of 2-3x10 (4)/cm(2) in a 96 well micro-fifer plate: Both albumin and glucose wer e added to each culture medium at final concentrations of 0-2 g/dl and 0-90 mM, respectively On days 2 and 3, 5-bromo-2'-deoxy-uridine (BrdU ) was added and incubated for 24-h to be incorporated into cellular DN A and detected by the ELISA principle; The lowest DNA synthesis was se en when both 2.0 g/dl of albumin and 90 mM of glucose: were: supplemen ted to the medium while the highest was obtained without albumin suppl ement. In the range of 0-90 mM of glucose and 0-2 g/dl of albumin, gen erally speaking, DNA synthesis of cultured mesothelial cells could be suppressed by either glucose or albumin, and it was greatly enhanced w hen both concentrations increased.