J. Nam et al., AGROBACTERIUM-TUMEFACIENS TRANSFORMATION OF THE RADIATION HYPERSENSITIVE ARABIDOPSIS-THALIANA MUTANTS UVH1 AND RAD5, Molecular plant-microbe interactions, 11(11), 1998, pp. 1136-1141
The Arabidopsis thaliana mutants uvh1 and rad5, originally identified
as radiation hypersensitive, were reported to be deficient in T-DNA in
tegration based on the relative efficiencies of stable transformation
and T-DNA transfer, We reassessed these mutants for susceptibility to
transformation by Agrobacterium tumefaciens. The mutant rad5 showed a
significant reduction in the efficiency of transient as well as stable
transformation, compared with its wild-type progenitor. These data in
dicate that rad5 is blocked at a step in the transformation process pr
ior to T-DNA integration. We additionally found, using both an in vitr
o root inoculation and an in vivo flower bolt inoculation assay, that
the mutant uvh1 is as susceptible to A, tumefaciens-mediated transform
ation as is its wild-type progenitor, C10.