EFFECT OF SORBIN DERIVATIVES ON CHOLERA TOXIN-INDUCED INTESTINAL SECRETION IN RAT IN-VIVO

The effect of synthetic sorbin derivatives was determined on cholera t oxin-stimulated jejunal secretion in anesthetized rats in vivo, using both perfused and ligated loop. An inhibitory effect on water secretio n induced by cholera toxin was shown with C-terminal sorbin peptides: C20 (YEPGKSSILQHERPVTKPQA-amide), C10 and Dala 7 heptapeptide-amide of sorbin, given by subcutaneous (SC) or intraduodenal administration. W hen perfused intravenously, C20-sorbin inhibited the cholera-induced s timulation of net Bur of water, Na+ and K+, in the jejunum and at the same time the net flux of water and Cl- in the duodenum, which was not in contact with the toxin. 5-hydroxytryptamine was not significantly changed in plasma or fluid. Prostaglandin E-2 release in jejunal as we ll as duodenal fluid was significantly stimulated by cholera toxin, bu t was not significantly different from basal value after C20 administr ation. (C) 1998 Elsevier Science Inc.