ANALYSIS OF GINGIVAL CREVICULAR FLUID INTRACYTOPLASMIC ENZYME-ACTIVITY IN PATIENTS WITH ADULT PERIODONTITIS AND RAPIDLY PROGRESSIVE PERIODONTITIS - A LONGITUDINAL-STUDY MODEL WITH PERIODONTAL TREATMENT

IN THE PRESENT STUDY, the activity of 3 functionally related enzymes, creatine kinase (CK), lactate dehydrogenase (LDH), and aspartate amino transferase (AST) levels in the rest and flow gingival crevicular flui d (rGCF, fGCF) from patients with rapidly progressive periodontitis (R PP) and adult periodontitis (AP) were determined before and after peri odontal treatment, including maintenance. When rGCF and fGCF mean enzy me levels were compared, rGCF was found to contain approximately twice as much enzyme levels than fGCF throughout the study. The findings of the present study revealed that both the rGCF and fGCF samples also c ontained higher CK, LDH, and AST levels than serum samples. Baseline c linical parameters and GCF enzyme levels presented a significant decli ne throughout the non-surgical and surgical treatment phases in both p atient groups, with surgical treatment being more effective. Despite c linical stability, in the AP group levels of LDH and AST showed a tend ency to increase in the third month, while enzyme levels still continu ed to decrease in the RPP group, who received additional antibiotics d uring the surgical phase. These findings suggest that GCF intracytopla smic enzyme profile is related with periodontal status and successful periodontal treatment, in addition to clinical improvement, has a sign ificant effect on this profile. Analysis of biochemical events, more s pecifically intracytoplasmic enzyme levels in GCE are likely to offer a sensitive measure of periodontal pathology which may help in overcom ing the existing limitations of clinical parameters. For this purpose, analysis of rGCF intracytoplasmic enzymes seems to be more beneficial .