Me. Sarciron et al., ALTERATIONS OF TOXOPLASMA-GONDII INDUCED BY 2',3'-DIDEOXYINOSINE IN-VITRO, The Journal of parasitology, 84(5), 1998, pp. 1055-1059
The time-course of action of the antiviral agent 2',3'-dideoxyinosine
(ddI) against Toxoplasma gondii tachyzoites in vitro and its effects a
t the ultrastructural level were investigated. The very short latency
of effect and high efficacy of ddI were evidenced by the fact that the
drugs' effects on parasite growth occurred 2 hr after addition to the
culture medium, and that an IL90 value of 0.5 mu g/ml was reached aft
er 72 hr. Although without apparent effect on uninfected cells, ddI cl
early acted on the intracellular parasites, which tended to disappear.
Remaining tachyzoites were almost exclusively extracellularly located
and often exhibited a clustering of mitochondria-like bodies and subs
equent deep alterations of their plasma membranes. These results confi
rm previous findings and emphasize the potential usefulness of ddI in
the management of cerebral toxoplasmosis, a major health problem in ac
quired immune deficiency syndrome patients.