THE ADVANCED GLYCATION END-PRODUCT N-EPSILON-(CARBOXYMETHYL)LYSINE ISINCREASED IN SERUM FROM CHILDREN AND ADOLESCENTS WITH TYPE-1 DIABETES

OBJECTIVE - To investigate whether children and adolescents with type 1 diabetes have increased serum levels of the glycoxidation product N- epsilon-(carboxymethyl)lysine (CML) at an early stage of the disease. RESEARCH DESIGN AND METHODS - The serum levels of CML in 38 patients w ith type 1 diabetes aged 14 +/- 3.2 (mean +/- SD) years were compared with those in 26 control subjects aged 16 +/- 1.7 years. The mean dura tion of diabetes was 5 +/- 4.7 years, ranging from 0.5 to 15 years. Th e mean levels of HbA(1c) were 10.3 +/- 2.5% in the patient group. The serum levels of CML were measured using a monoclonal anti-CML antibody in a fluoremetric immunoassay. Serum protein levels of advanced glyca tion end products (AGEs) were assayed using a polyclonal antibody from rabbit immunized with AGE-RNase (pAGE). RESULTS - The serum levels of CML and pAGE were significantly increased in the patient group versus the control group: 1.08 (0.45-2.97) U/ml CML (median 10-90 percentile s) vs. 0.70 (0.36-1.79) U/ml CML, P < 0.03, and 6.6 (5.1-9.9) U/ml pAG E vs. 5.5 (3.7-8.2) U/ml AGEs, P < 0.01. A significant relationship be tween CML and pAGE was found in the IDDM group, r = 0.76, P < 0.001. T he CML levels were not associated with the HbA(1c) levels (n = 23, r = -0.02, NS), cholesterol levels (n = 21, r = 0.07, NS), age, sex, or d iabetes duration. CONCLUSIONS - Serum levels of CML are increased in p atients with type 1 diabetes. This increase precedes the development o f micro- and macrovascular complications.