Md. Desmet et al., A NOVEL METHOD FOR THE DETERMINATION OF T-CELL PROLIFERATIVE RESPONSES IN PATIENTS WITH UVEITIS, Ocular immunology and inflammation, 6(3), 1998, pp. 173-178
Standard proliferation assays using autoantigens such as S-Ag have giv
en erratic responses when studied with human peripheral blood mononucl
ear cells. This erratic response is a reflection of the low number of
circulating cells in the peripheral blood capable of generating a resp
onse as well as the presence of competing cells for the available cyto
kines in culture. The present study compares the standard proliferatio
n assay with a novel technique in which multiple short-term cell lines
are established to S-Ag in medium enriched in helper cytokines. After
12-14 days of culture, these lines were tested for their response to
S-Ag. A significant difference was found between patients and controls
in the ability to generate responsive cell lines. This translated to
a frequency of responsive cells of 0-4 per 10(7) peripheral blood mono
nuclear cells (PBMC) in normal individuals and 0-200 per 10(7) PBMC in
patients. This novel technique may provide a means of determining the
number of responsive cells to specific autoantigens in the peripheral
blood of patients and the ability to follow the response over time.