SYNERGISTIC EFFECTS OF FOCUS ULTRASOUND WITH DIFFERENT FREQUENCY AND HEMATOPORPHYRIN ON HUMAN TUMOR-CELLS

Human hematopoietic cell K-562. human melenoma cell LiBr and human sto mach cancer cells were exposed to ultrasound (US, 1.75 W/cm(2), 1.4, 2 .16 and 2.4 MHz) in vitro in the presence or absence of hematoporphyri n (Hp, 100 mu g/mL). The cell damaging effects of treatments were dete rmined by means of the Trypan Blue dye exclusion test, MTT test and FD A test. The experimental results showed that the same cell line had di fferent sensibilities to the US of different frequencies, and differen t cell line had different damage at the same acoustical radiation. The combined treatment with US and Hp enhanced greatly the cell damage, a nd ilo sensibility oi insonation cells to US with Hp was observed. The cell damage rests showed that the results of MTT test corresponded we ll with that of Trypan Blue dye test.