LIQUID-CHROMATOGRAPHIC METHOD FOR THE ANALYSIS OF ALL-RAC-ALPHA-TOCOPHERYL ACETATE AND RETINYL PALMITATE IN SOY BASED INFANT FORMULA USING MATRIX SOLID-PHASE DISPERSION

A liquid chromatographic method is described for the analysis of all-r ac-alpha-tocopheryl acetate and retinyl palmitate in soy based infant formula. The vitamins are extracted from infant formula without saponi fication by matrix solid phase dispersion (MSPD) and quantitated by no rmal phase chromatography with fluorescence detection. Retinyl palmita te and all-rac-alpha-tocopheryl acetate are quantitated isocratically with a mobile phase of hexane containing isopropanol @ 0.125% (v/v) an d 0.5% (v/v), respectively. Results compared favorably to the label de claration on a retail infant formula product.