HSCCC SEPARATION OF A STILBENE GLUCOSIDE FROM POLYGONUM-MULTIFLORUM

Authors
CAO XL TIAN Y ZHANG TY SHEN PN ZHU LH ITO Y
Citation
Xl. Cao et al., HSCCC SEPARATION OF A STILBENE GLUCOSIDE FROM POLYGONUM-MULTIFLORUM, Journal of liquid chromatography & related technologies, 21(18), 1998, pp. 2897-2904
Citations number
8
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of liquid chromatography & related technologies → ACNP
ISSN journal
10826076
Volume
21
Issue
18
Year of publication
1998
Pages
2897 - 2904
Database
ISI
SICI code
1082-6076(1998)21:18<2897:HSOASG>2.0.ZU;2-1
Abstract
High-speed countercurrent chromatography (HSCCC) was applied to the se paration and purification of 2,3,5,4'-tetrahydroxy stilbene-2-O-D-gluc oside from dried roots (2.7g) of Polygonum multiflorum. The ethanol ex tract (240 mg) was first separated with the solvent system composed of ethyl acetate-ethanol-water at a volume ratio of 10:1:10 and the obta ined fraction was repurified with the solvent system at the modified v olume ratio of 50:1:50, The method produced stilbene glucoside (30mg) at a high purity of 96%. The chemical structure was identified by FAB- MS and (HNMR)-H-1.