OPTICAL-PROPERTIES OF BOVINE MUSCLE-TISSUE IN-VITRO - A COMPARISON OFMETHODS

We measured the optical properties of muscular tissue using several me thods. Collimated transmission measurements of thin slabs showed spati al anisotropy of the scattering processes. Surface roughness of the sa mple disables the calculation of the extinction coefficient from these measurements. From angular intensity measurements we found a scatteri ng asymmetry parameter g = 0.96. In fresh samples the optical diffusio n constant D depends on the orientation with respect to the longitudin al direction of the muscular cells. From the D values we calculated s' perpendicular to the longitudinal direction as 0.19 mm(-1) (at 543 nm ), 0.39 mm(-1) (at 594 nm) and 0.59 mm(-1) (at 632 nm). The values for D which we measured from samples that were frozen and thawed did not show dependence on orientation. From spectral dependent reflectance me asurements we found an oxygenation degree of 0.61 and a reduced scatte ring coefficient s' = 0.85 mm(-1) around 560 nm.