A 7.4-ANGSTROM PROJECTION STRUCTURE OF OUTER-MEMBRANE PHOSPHOLIPASE-AFROM ESCHERICHIA-COLI BY ELECTRON CRYSTALLOGRAPHY

Authors
BOEKEMA EJ STUART M KONING RI KEEGSTRA W BRISSON A VERHEIJ HM DEKKER N
Citation
Ej. Boekema et al., A 7.4-ANGSTROM PROJECTION STRUCTURE OF OUTER-MEMBRANE PHOSPHOLIPASE-AFROM ESCHERICHIA-COLI BY ELECTRON CRYSTALLOGRAPHY, Journal of structural biology (Print), 123(1), 1998, pp. 67-71
Citations number
20
Categorie Soggetti
Biophysics,Biology,"Cell Biology
Journal title
Journal of structural biology (Print) → ACNP
ISSN journal
10478477
Volume
123
Issue
1
Year of publication
1998
Pages
67 - 71
Database
ISI
SICI code
1047-8477(1998)123:1<67:A7PSOO>2.0.ZU;2-A
Abstract
Outer membrane phospholipase A (OMPLA) is one of the few enzymes prese nt in the outer membrane of Escherichia coli. Two-dimensional crystals of OMPLA were grown by reconstitution of purified protein into lipid bilayers via detergent dialysis and were studied by electron crystallo graphy. A 7.4-Angstrom projection map reveals OMPLA molecules exhibiti ng an oval-shaped domain of 30 x 20 Angstrom resembling the beta-barre l structure characteristic of porins, which is associated with a 25-An gstrom elongated domain of lower density. (C) 1998 Academic Press.