SYNTHESIS AND BINDING STUDY OF PHOSPHONATE ANALOGS OF PNAS AND THEIR HYBRIDS WITH PNAS

The preparation of monomers for time synthesis of phosphonate analogue s of peptide nucleic acids containing the four natural nucleobases: th ymine, cytosine, adenine and guanine, has been ccomplished. The monome rs obtained were used for the automated online solid phase synthesis o f pure phosphono-PNA oligomers as well as chimeras consisting of phosp hono-PNA and]PNA resudies. The hybridization properties of these oligo nucleotide mimics to complementary DNA and RNA fragments were studied.