FLUORESCENCE RESONANCE ENERGY-TRANSFER (FRET) TO FOLLOW RIBOZYME REACTIONS IN REAL-TIME

Authors
HANNE A RAMANUJAM MV RUCKER T KRUPP G
Citation
A. Hanne et al., FLUORESCENCE RESONANCE ENERGY-TRANSFER (FRET) TO FOLLOW RIBOZYME REACTIONS IN REAL-TIME, Nucleosides & nucleotides, 17(9-11), 1998, pp. 1835-1850
Citations number
7
Categorie Soggetti
Biology
Journal title
Nucleosides & nucleotides → ACNP
ISSN journal
07328311
Volume
17
Issue
9-11
Year of publication
1998
Pages
1835 - 1850
Database
ISI
SICI code
0732-8311(1998)17:9-11<1835:FRE(TF>2.0.ZU;2-B
Abstract
Fluorescence resonance transfer (FRET) was applied far real time monit oring of ribozyme reactions. Group I ribozyme ligation was followed wi th two separate, fluorescent-labeled RNA substrates. For hammerhead ri bozyme cleavage, a double-fluorescent-labeled substrate was used. For the first time we analyzed multiple turnover conditions. Real time mon itoring permits convenient analysis of ribozyme kinetics and the seque nce-specific, quantitative detection of RNAs in femtomole amounts.