POSSIBILITIES OF TRIPLEX-FORMATION BY 21-MER OLIGODEOXYRIBONUCLEOTIDES AS ANALOGS OF TETRAHYMENA PRE-RIBOSOMAL-RNA FRAGMENTS

A potential DNA triple helix of 21-mer oligodeolryribonucleotides was synthesized and characterized. The strands were chosen to study the in teraction of internal guide and intervening sequences analogs as well as adjacent 3' and 5' exon parts around the splicing site of Tetrahyme na pre-rRNA. Further in parallel works a series of different RNA and D NA strands was synthesized and combined yielding a suitable order of s tability. Here we want to show an isolated examination of a DNA-strand triple helix with defined sequences containing a central mismatched b ase arrangement and T-A-T bases at the ends.