THE ACCURATE DETECTION OF ONE-POINT MUTATIONS BY LIGATION OF SHORT OLIGONUCLEOTIDES

Authors
ZARYTOVA VF PYSHNYI DV KRIVENKO AA DYMSHITZ GM IVANOVA EM
Citation
Vf. Zarytova et al., THE ACCURATE DETECTION OF ONE-POINT MUTATIONS BY LIGATION OF SHORT OLIGONUCLEOTIDES, Nucleosides & nucleotides, 17(9-11), 1998, pp. 2143-2147
Citations number
7
Categorie Soggetti
Biology
Journal title
Nucleosides & nucleotides → ACNP
ISSN journal
07328311
Volume
17
Issue
9-11
Year of publication
1998
Pages
2143 - 2147
Database
ISI
SICI code
0732-8311(1998)17:9-11<2143:TADOOM>2.0.ZU;2-Z
Abstract
A new approach which is based on the selective ligation of the coopera tive tandem of three short oligonucleotides (octa-, tetra- and octamer s) is proposed to reveal a point mutation in DNA. The ligation product is registered only when the correct complex of the tandem and DNA is formed. If there is one substitution base in the binding site of tetra nucleotide in DNA, no product of ligation is observed.