Sf. Penc et al., DERMATAN SULFATE RELEASED AFTER INJURY IS A POTENT PROMOTER OF FIBROBLAST GROWTH FACTOR-2 FUNCTION, The Journal of biological chemistry, 273(43), 1998, pp. 28116-28121
Proteoglycans have been shown in vitro to bind multiple components of
the cellular microenvironment that function during wound healing. To s
tudy the composition and function of these molecules when derived from
an in vivo source, soluble proteoglycans released into human wound fl
uid were characterized and evaluated for influence on fibroblast growt
h factor-2 activity. Immunoblot analysis of wound fluid revealed the p
resence of syndecan-1, syndecan-4, glypican, decorin, perlecan, and ve
rsican. Sulfated glycosaminoglycan concentrations ranged from 15 to 65
mu g/ml, and treatment with chondroitinase B showed that a large prop
ortion of the glycosaminoglycan was dermatan sulfate. The total glycos
aminoglycan mixture present in wound fluid supported the ability of fi
broblast growth factor-a to signal cell proliferation. Dermatan sulfat
e, and not heparan sulfate, was the major contributor to this activity
, and dermatan sulfate bound FGF-2 with K-d = 2.48 mu M. These data de
monstrate that proteoglycans released during wound repair are function
ally active and provide the first evidence that dermatan sulfate is a
potent mediator of fibroblast growth factor-2 responsiveness.