M. Alvarezguerra et al., THE ERYTHROCYTE NA,K,CL COTRANSPORTER AND ITS CIRCULATING INHIBITOR IN DAHL SALT-SENSITIVE RATS, Journal of hypertension, 16(10), 1998, pp. 1499-1504
Background Abnormal Na,K,Cl cotransport is thought to be a pathogenic
factor in Dahl salt-sensitive rat models, but the only direct evidence
for this is an increased cotransport activity found in erythrocytes f
rom salt-loaded Dahl salt-sensitive rats. Objective To re-examine eryt
hrocyte cotransport fluxes and a circulating cotransport inhibitory fa
ctor (CIF) in inbred Dahl rats maintained on a low (0.2%) salt diet Co
transport fluxes were investigated both under basal conditions and aft
er stimulation by cell shrinking. Methods Blood was drawn from 12 male
Dahl salt-sensitive and 12 Dahl salt-resistant rats of the inbred Joh
n Rapp strain. Erythrocyte Na,K,Cl cotransport activity was equated to
the bumetanide-sensitive fluxes of sodium, rubidium or lithium. Plasm
a CIF activity was tested in human erythrocytes. Results In Dahl salt-
sensitive rats: (1) plasma CIF activity (5.7 +/- 0.4 units/ml) was mod
estly higher than in Dahl salt-resistant rats (2.97 +/- 0.12 units/ml,
P < 0.0001), but much lower than that previously found in salt-loaded
Dahl salt-sensitive rats (16.1 units/ml), and (2) erythrocytes exhibi
ted a similar bumetanide-sensitive sodium efflux (rate constant 0.056
+/- 0.008 h(-1)) as in Dahl salt-resistant rats (0.047 +/- 0.007 h(-1)
). Following hypertonic shock, the bumetanide-sensitive rubidium influ
x reacted more to cell shrinkage in Dahl salt-sensitive than in Dahl s
alt-resistant erythrocytes (cell volume decrease required to stimulate
bumetanide-sensitive rubidium influx by 4000 mu mol/l cells per h = -
4.04 +/- 0.36 versus -5.89 +/- 0.44 fl, respectively; P < 0.01). Concl
usions When fed a low-salt diet, Dahl salt-sensitive rats present slig
htly increased plasma CIF levels and normal erythrocyte cotransport fl
uxes under basal conditions, but an increased response to a hypertonic
shock. Therefore, if there is any primary cotransport abnormality in
Dahl salt-sensitive rats, it appears to be restricted to the renal Na,
K,Cl cotransporter BSC1 isoform. Alternatively, any such change may be
the consequence of abnormal regulation by osmolarity-dependent mechan
isms. (C) 1998 Lippincott Williams & Wilkins.