RECENT ADVANCES IN SITE-DIRECTED SPIN-LABELING OF PROTEINS

Site-directed spin labeling of proteins is experiencing a phase of rap id technical evolution, application and evaluation, New strategies hav e been introduced for determining membrane protein topography, electro static potentials, the orientation of proteins at membrane surfaces an d inter-residue distances, New applications include studies of beta st rands, structure mapping using spin-spin interactions, domain motions in soluble proteins and extensive structural analysis of a number of m embrane and soluble proteins.