SINGLE-SEED PCR OF LMW GLUTENIN GENES TO DISTINGUISH BETWEEN DURUM-WHEAT CULTIVARS WITH GOOD AND POOR TECHNOLOGICAL PROPERTIES

Polymerase chain reaction (PCR) was used to amplify low-molecular-weig ht (LMW) glutenin sequences from genomic DNA extracted from a single g erminating seed of several durum wheat genotypes. Electrophoretic anal ysis of PCR reactions showed the presence of amplified products charac teristic of durum wheat cultivars with good and poor technological pro perties. This PCR-based approach is proposed as a very efficient and s afe alternative to standard procedures for selecting durum wheat genot ypes with good qualitative characteristics.