CHLORINA AND VIRIDIS MUTANTS OF BARLEY (HORDEUM-VULGARE L.) ALLOW ASSIGNMENT OF LONG-WAVELENGTH CHLOROPHYLL FORMS TO INDIVIDUAL LHCA PROTEINS OF PHOTOSYSTEM-I IN-VIVO

The isolated subcomplex LHCI-730 of plant photosystem I(PSI) chlorophy ll (Chl) alb binding antenna is a heterodimer of Lhca1 and Lhca4 and h as a 77 K fluorescence emission peak at 730 nm (F730), Recently, three Chi spectral forms with 77 K fluorescence emission peaks at 720 nm, 7 30 nm and 742 nm were identified in native PSI, In an attempt to assig n the two longest wavelength emission maxima to peripheral PSI antenna proteins, we performed 77 K fluorescence emission spectroscopy on int act leaves of chlorina and viridis mutants from barley which lack indi vidual LHCI-730 proteins. This approach indicates that F732 is found o nly in Lhca1 and F742 only in Lhca4, when these proteins are associate d with the PSI reaction centre. (C) 1998 Federation of European Bioche mical Societies.