SEXING OF HUMAN AND OTHER PRIMATE DNA

Authors
WILSON JF ERLANDSSON R
Citation
Jf. Wilson et R. Erlandsson, SEXING OF HUMAN AND OTHER PRIMATE DNA, Biological chemistry, 379(10), 1998, pp. 1287-1288
Citations number
11
Categorie Soggetti
Biology
Journal title
Biological chemistry → ACNP
ISSN journal
14316730
Volume
379
Issue
10
Year of publication
1998
Pages
1287 - 1288
Database
ISI
SICI code
1431-6730(1998)379:10<1287:SOHAOP>2.0.ZU;2-P
Abstract
We present a rapid and simple method for the sexing of human and other primate DNA, Homologous introns from the X- and Y-linked zinc finger protein genes (ZFX and ZFY) are PCR amplified with one primer pair. An Alu element insertion in the ZFX intron results in a size difference in the amplicons which is resolved by agarose gel electrophoresis.