SYNTHESIS OF PHOSPHORAMIDE MUSTARD ANALOGS OF DAUNOMYCIN AND CARMINOMYCIN

New phosphoramide mustards (6-8) have been prepared from the antibioti cs 2 and 3, and from 5. The mixture of cyclophosphamides could be sepa rated by preparative layer and column chromatography. The assignments of configuration to the isomeric phosphoramidates was based on the mag netic anisotropy of the P=O bond. The synthesized compounds 6a,b-8a,b (mixture of isomers) were tested for inhibitory activity on the [H-3]- thymidine incorporation to the DNA of tumor cells, using ovarian carci noma cell line.