The involvement of kinases in heat stress signaling in tomato cells wa
s studied by in gel kinase assays using myelin basic protein as substr
ate, and by in vitro phosphorylation assays in Mono Q fractions of tom
ato cell lysates. A kinase with an apparent molecular mass of approxim
ately 50 kDa is rapidly deactivated upon heat stress as judged from in
gel kinase assays. Cycloheximide treatment increases kinase activity,
but concomitant heat treatment abolishes cycloheximide-induced activa
tion. Kinase activity from untreated cells was recovered at about 130
and 250 mM NaCl from Mono Q columns.