STUDIES ON THE STABILITY AND CONFORMATION OF MONOACYLATED ANTHOCYANINS PART 2 - ANTHOCYANINS FROM WILD CARROT SUSPENSION-CULTURES ACYLATED WITH SUPPLIED CARBOXYLIC-ACIDS

The anthocyanins accumulated by carrot cell cultures include some that are acylated. Addition of cinnamic and benzoic acid analogues to the culture medium resulted in the production of fourteen novel monoacylat ed anthocyanins. Four additional anthocyanins were prepared by partial acid hydrolysis, which selectively removed the xylose side chain from the branched trisaccharide part of the molecule. The carrot cells acc ommodate a wide range of carboxylic acids used to acylate anthocyanins and provide a system for the preparation of anthocyanins that have a variety of acyl groups attached. The compounds were characterized by H -1 NMR spectroscopy and mass spectrometry, and by UV-vis absorption sp ectroscopy at both pH 2 and 6. (C) 1998 Elsevier Science Ltd. All righ ts reserved.