A new class of recessive Arabidopsis mutants, designated cristal (cii)
has been isolated which display several abnormalities reminiscent of
hyperhydric symptoms. These characteristics include translucent and wr
inkled cotyledons and leaves, abnormal chloroplast organization, a red
uced amount of chlorophyll, a reduced dry weight and a decreased numbe
r of palisade cells in the leaves accompanied by an increase of interc
ellular space, and therefore give a vitreous appearance to the aerial
part. The phenotype is also dependent on the culture medium water pote
ntial. The cril gene was mapped on chromosome 4 close to the DHS1 mark
er.