THE IMPORTANCE OF GLU(361) POSITION IN THE REACTION CATALYZED BY CHOLESTEROL OXIDASE

Authors
KASS IJ SAMPSON NS
Citation
Ij. Kass et Ns. Sampson, THE IMPORTANCE OF GLU(361) POSITION IN THE REACTION CATALYZED BY CHOLESTEROL OXIDASE, Bioorganic & medicinal chemistry letters, 8(19), 1998, pp. 2663-2668
Citations number
19
Categorie Soggetti
Chemistry Inorganic & Nuclear","Chemistry Medicinal
Journal title
Bioorganic & medicinal chemistry letters → ACNP
ISSN journal
0960894X
Volume
8
Issue
19
Year of publication
1998
Pages
2663 - 2668
Database
ISI
SICI code
0960-894X(1998)8:19<2663:TIOGPI>2.0.ZU;2-V
Abstract
Cholesterol oxidase stereospecifically isomerizes cholest-5-en-3-one t o cholest-4-en-3-one. When the base catalyst for isomerization, Glu(36 1), is mutated to Asp, the rate of deprotonation of cholest-5-en-3-one is not affected, but protonation of the dienolic intermediate becomes rate-limiting. This may be a consequence of the large distance betwee n the catalytic base and carbon-6 of the intermediate in the mutant en zyme. (C) 1998 Elsevier Science Ltd. All rights reserved.