CHARACTERIZATION OF STRESS-RESPONSIVE BEHAVIORS OF PROTEINS

Protein denaturation processes under stress conditions such as pH, den aturant and heat are evaluated by employing several approaches such as aqueous two-phase partitioning and circular dichroism (CD) measuremen t in order to determine the conformational states and surface properti es of proteins, It Is suggested that the manner of conformational chan ge of protein from the native state to the molten globule one does not depend on these sorts of stresses if both the strength of stress and surface properties of proteins are normalized between the above two st ates. By using these normalization parameters, eight proteins used in this study mere simply divided into two groups namely monomeric protei ns and oligomeric one. On the other hand, the strength of stress which inducing protein denaturation up to the molten globule state greatly depends on the sort of stresses or protein species. From these conside rations, the possibility of using effective stress-mediated separation process of protein is suggested as an application of the analysis of the stress responsive behaviors of proteins.