MICROSATELLITE MARKERS IN COMMON CARP (CYPRINUS-CARPIO L)

Microsatellite markers of the poly (CA) type in common carp (Cyprinus carpio L.) are described. Clones containing a (CA) repeat were isolate d from a common carp genomic library and sequenced. The number of repe ats found was high compared to mammals but comparable with other teleo st fishes. Classification of the repeats (perfect, imperfect and compo und) are compared with the Atlantic cod (Gadus morhua L.), rainbow tro ut (Oncorhynchus mykiss), and Atlantic salmon (Salmo salar L.). A tota l of 41 primer sets were designed and tested for polymorphism on a tes t panel of eight animals (derived from outbred lines, inbred lines and gynogenetic clones). Thirty-two markers were found to be polymorphic. The heterozygosity in the outbred animals was 60.4%, 51.1% in the inb red animals and 0% in the gynogenetic clones. The average number of al leles among the eight animals was 4.7 per marker. Six markers (18.8%) gave an additional polymorphic amplification product besides the polym orphic amplification product in the expected size range. The possibili ty that these loci are tetraploid is discussed. The polymorphic loci d escribed for common carp will be valuable as genetic markers for use i n population, breeding, and evolutionary studies.