FAD-MEDIATED ENZYMATIC CONVERSION OF NAD(- APPLICATION TO CHIRAL SYNTHESIS OF L-LACTATE() TO NADH )

Authors
LEONIDA MD SOBOLOV SB FRY AJ
Citation
Md. Leonida et al., FAD-MEDIATED ENZYMATIC CONVERSION OF NAD(- APPLICATION TO CHIRAL SYNTHESIS OF L-LACTATE() TO NADH ), Bioorganic & medicinal chemistry letters, 8(20), 1998, pp. 2819-2824
Citations number
11
Categorie Soggetti
Chemistry Inorganic & Nuclear","Chemistry Medicinal
Journal title
Bioorganic & medicinal chemistry letters → ACNP
ISSN journal
0960894X
Volume
8
Issue
20
Year of publication
1998
Pages
2819 - 2824
Database
ISI
SICI code
0960-894X(1998)8:20<2819:FECONA>2.0.ZU;2-U
Abstract
Electroenzymatic reduction of NAD(+) to NADH for subsequent use in enz ymatic synthesis has been carried out at carbon electrodes bearing lip oamide dehydrogenase (LiDH) immobilized under a Nafion(R) film. The se lf-mediated electron transfer was made possible by an excess of flavin adenine dinucleotide (FAD) entrapped together with LiDH. Results were compared to those obtained with a similar electrode containing both L iDH and a polymeric form of FAD (pFAD) prepared by anodic polymerizati on of FAD. (C) 1998 Elsevier Science Ltd. All rights reserved.