Ca. Opere et Se. Ohia, ROLE OF INTRACELLULAR CALCIUM IN PEROXIDE-INDUCED POTENTIATION OF NOREPINEPHRINE RELEASE FROM BOVINE ISOLATED IRIDES, General pharmacology, 31(5), 1998, pp. 793-798
1. BAPTA AM (10 mu M), thapsigargin (10 mu M), ruthenium red (30 mu M)
and oligomycin (30 mu M) inhibited field stimulated [H-3]NE release f
rom bovine isolated irides by 54%, 30%, 30% and 26%, respectively. 2.
Both BAPTA AM and thapsigargin had no significant effect (P > 0.05) on
H2O2-induced potentiation of evoked [H-3]NE release. 3. Ruthenium red
prevented (but oligomycin enhanced) H2O2-induced enhancement of evoke
d [H-3]NE release. 4. We conclude that, although intracellular calcium
participates in field-stimulation evoked [H-3]NE release from bovine
isolated irides, only the mitochondrial pool of calcium may be involve
d in peroxide-induced enhancement of sympathetic neurotransmission. (C
) 1998 Elsevier Science Inc.