HOMOLOGY BETWEEN HUMAN-CHROMOSOME 2P13.3 AND THE WOBBLER CRITICAL REGION ON MOUSE-CHROMOSOME-11 - COMPARATIVE HIGH-RESOLUTION MAPPING OF STS AND EST LOCI ON YAC BAC CONTIGS/

Human Chr 2p13-14 and homologous regions on mouse Chrs 6 and 11 have b een subjects of previous studies because they comprise the loci for se veral neuromuscular diseases. Here we report on high-resolution mappin g of 55 STS and EST loci on human Chr 2p13.3 and of 47 markers on the corresponding region on proximal mouse Chr. ii. The maps comprise seve ral known genes, MEIS1/Meis1, RAB1a/Rab1a, MDH1/Mor2, OTX1/Otx1, and R EL on human 2p13.3 and mouse Chr II, respectively, as well as the wobb ler (wr) critical region of the mouse. Whereas a perfect correspondenc e was found in most of the 4-Mb region, a small rearrangement was disc overed around the OTX1/Otx1 locus. The detailed STS and EST transcript maps of these regions and a further narrowing down of the mouse wr cr itical region to the interval between D11Mit79 and D11Mit19 allow for the selection of positional candidate genes for wr, and the exclusion of others.