ENDONUCLEASE SEGE OF PHAGE-T4 .2. RECOGNITION SITE

Authors
KRYUKOV VM SHLYAPNIKOV MG KADYROV FA
Citation
Vm. Kryukov et al., ENDONUCLEASE SEGE OF PHAGE-T4 .2. RECOGNITION SITE, Molecular biology, 30(6), 1996, pp. 786-791
Citations number
31
Categorie Soggetti
Biology
Journal title
Molecular biology → ACNP
ISSN journal
00268933
Volume
30
Issue
6
Year of publication
1996
Part
1
Pages
786 - 791
Database
ISI
SICI code
0026-8933(1996)30:6<786:ESOP.R>2.0.ZU;2-N
Abstract
Phage T4 site-specific endonuclease SegE cleaves dsDNA with formation of 2-nt 3'-protruding ends. The minimal recognized sequence is asymmet rical: positions -1 to +17 (+19) relative to the cleavage point center . The SegE recognition site is highly degenerate, and cannot be unambi guously predicted from the DNA primary structure.