DETERMINATION OF GLUTAMATE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUORESCENCE DETECTION IN SUPERFUSATES OF RAT-BRAIN PREPARATIONS

Authors
IOANNOU N GEORGOPOULOS A LIAPI C PALAIOLOGOS G
Citation
N. Ioannou et al., DETERMINATION OF GLUTAMATE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUORESCENCE DETECTION IN SUPERFUSATES OF RAT-BRAIN PREPARATIONS, Analytical biochemistry (Print), 264(1), 1998, pp. 82-86
Citations number
5
Categorie Soggetti
Biology,"Biochemical Research Methods","Chemistry Analytical
Journal title
Analytical biochemistry (Print) → ACNP
ISSN journal
00032697
Volume
264
Issue
1
Year of publication
1998
Pages
82 - 86
Database
ISI
SICI code
0003-2697(1998)264:1<82:DOGBHL>2.0.ZU;2-B
Abstract
A method is described for the determination of glutamate in superfusat es of cortical or hippocampal rat brain slices. This method is based o n the precolumn derivatization of amino acids with orthopthaldialdehyd e and mercaptopropionic acid, separation by highperformance liquid chr omatography, and detection by fluorescence. By adjusting the concentra tions of reagents and with the minimum dilution of the sample, it was possible to reproducibly measure the Ca2+-dependent K+-evoked release of neurotransmitter glutamate in superfusates of brain slices. (C) 199 8 Academic Press.