SEPARATION OF CAROTENOIDS BY SUBCRITICAL FLUID CHROMATOGRAPHY WITH COATED, PACKED CAPILLARY COLUMNS AND NEAT CARBON-DIOXIDE

Citation
E. Ibanez et al., SEPARATION OF CAROTENOIDS BY SUBCRITICAL FLUID CHROMATOGRAPHY WITH COATED, PACKED CAPILLARY COLUMNS AND NEAT CARBON-DIOXIDE, Journal of chromatography, 823(1-2), 1998, pp. 313-319
Citations number
20
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Volume
823
Issue
1-2
Year of publication
1998
Pages
313 - 319
Database
ISI
SICI code
Abstract
Different packed capillary columns were evaluated for carotenoid separ ation by subcritical fluid chromatography with neat CO2. Packed capill ary columns studied involved the use of silica particles deactivated w ith different methods and particles coated with stationary phases comm only used in gas chromatography, such as SE-54 (95% methyl-, 5% phenyl silicone) and OV-17 (50% methyl-, 50% phenylsilicone). In order to sel ect the appropriate chromatographic conditions to elute carotenoids, a theoretical study of the solubility of beta-carotene was performed. S eparation of lycopene and beta-carotene was obtained with neat CO2 wor king at pressures around 300 atm and at a temperature of 10 degrees C (1 atm=101 325 Pa). Evaluation of the columns used in the present stud y in terms of efficiency and activity was also performed. (C) 1998 Els evier Science B.V. All rights reserved.