Sm. Pons et al., ANALYSIS OF HUMAN-MILK TRIACYLGLYCEROLS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH LIGHT-SCATTERING DETECTION, Journal of chromatography, 823(1-2), 1998, pp. 475-482
Citations number
39
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
A high-performance liquid chromatography (HPLC) method for the separat
ion of human milk triacylglycerols using a C-18 Spherisorb ODS column
and ternary gradient elution with dichloromethane, acetone and acetoni
trile is described. The triacylglycerols are detected by light scatter
ing. Several chromatographic conditions were assayed in order to optim
ize the method: sample solubility, mobile phase, column temperature an
d the mass detector oven temperature. The linearity, precision and rel
ative response of the method were examined. A total of 34 peaks were s
eparated and quantified based on the percentage peak area in the HPLC
chromatogram. Mature human milk analyzed by this method contained six
predominant triacylglyceride structures: POO, POL, LOO, POP, OOO and S
OP, where P=palmitin, O=olein, L=linolein and S=stearin. (C) 1998 Else
vier Science B.V. All rights reserved.