ONLINE COUPLING OF MICRODIALYSIS SAMPLING WITH LIQUID-CHROMATOGRAPHY FOR THE DETERMINATION OF PEPTIDE AND NONPEPTIDE LEUKOTRIENES

Authors
KJELLSTROM S EMNEUS J LAURELL T HEINTZ L MARKOVARGA G
Citation
S. Kjellstrom et al., ONLINE COUPLING OF MICRODIALYSIS SAMPLING WITH LIQUID-CHROMATOGRAPHY FOR THE DETERMINATION OF PEPTIDE AND NONPEPTIDE LEUKOTRIENES, Journal of chromatography, 823(1-2), 1998, pp. 489-496
Citations number
20
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography → ACNP
Volume
823
Issue
1-2
Year of publication
1998
Pages
489 - 496
Database
ISI
SICI code
Abstract
An automated on-line sampling method was developed using microdialysis as the simultaneous sampling and sample pre-treatment technique. The extraction fraction values of microdialysis probes sampling different eicosanoids were investigated. The impact of cyclodextrins in the perf usion liquid used for sampling hydrophobic eicosanoids in biological s ystems was also studied. The total time for one analysis was 7.6 min a llowing seven measurements per hour for monitoring kinetic changes in biological systems. (C) 1998 Elsevier Science B.V. All rights reserved .