SEPARATION OF TOSYLATED POLYAMINES BY HIGH-PERFORMANCE CAPILLARY-ZONE-ELECTROPHORESIS

The successful separation of tosylated polyamines putrecine (PUT), cad averine (CAD) spermidine (SPD) and spermine (SPM) by high-performance capillary zone electrophoresis in an uncoated fused-silica capillary i s reported. The optimum conditions for obtaining the resolution betwee n PUT and CAD was 5 mM sodium phosphate buffer (pH 2.2) containing 0.8 7 mM SPD as an electroosmotic flow modifier, with a gradient voltage t hat starts at 10 kV and then jumps to 30 kV in different steps. The us e of different injection volumes is also discussed. (C) 1998 Elsevier Science B.V. All rights reserved.