QUANTITATIVE-ANALYSIS OF SPERMATOGENESIS IN RATS MADE AZOOSPERMIC WITH COMPOUND CDRI 84 35/

Compound CDRI 84/35 (1-formyl-4-dichloroacetamidopiperazine) was repor ted to exert its effect on seminiferous epithelium without affecting L eydig cells and accessory sex organs. Adult rats administered CDRI 84/ 35 (100 mg/kg body weight for 15 days followed by 25 mg/kg) showed a s ignificant atrophy of testis indicating 21.8 +/- 4.4, 79.6 +/- 5.3, 94 .8 +/- 2.1 and 79.5 +/- 2.1% abnormal tubules at 22, 41, 54, and 64 da ys following treatment at the time of autopsy). The Sertoli cell-germ cell ratio showed a significant reduction in number of primary spermat ocytes and spermatids during the treatment. Marked decrease in pachyte ne spermatocytes and in stages of spermatogenesis was observed on day 54. In contrast, the testes in estradiol benzoate (EB; 5 g/rat/day) tr eated rats showed 64.6% +/- 21.85% abnormal tubules at day 22 and almo st all affected tubules on from day 41 onwards, exhibiting a significa nt decline in number of spermatocytes and spermatids, while spermatogo nia were affected only at 64 days of treatment. Reversibility studies showed 76.5% +/- 2.2% normal tubules with significant inhibition in sp ermatogenesis following 60 days cessation of CDRI 84/35. At 120 days r ecovery, 74.7% +/- 28.8% testicular tubules revealed quantitatively no rmal spermatogenesis, whereas 25.3% +/- 18.8% tubules showed incomplet e recovery of spermatogenesis until 120 days. The present study reveal ed a marked inhibition of spermatogenesis at the pachytene spermatocyt e stage by CDRI 84/35 compared to EB in the seminiferous epithelium of rat. Its antispermatogenic effect was found to be irreversible up to 120 days. (C) 1998 Elsevier Science Inc. All rights reserved.